Gender:Male
Date of Birth:1977-04-06
Alma Mater:Peking University
Education Level:Postgraduate (Doctoral)
[MORE] Academic Honor:2021 Winner of National Science Fund for Distinguished Young Scholars
北京大学黄廷方/信和青年杰出学者奖
博雅青年学者奖
Impact Factor:9.6
DOI number:10.1073/pnas.1614614113
Journal:Proc Natl Acad Sci U S A
Abstract:The proteasome is the major engine of protein degradation in all eukaryotic cells. At the heart of this machine is a heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitylated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides. Using cryoelectron microscopy, we determined a near-atomic-resolution structure of the 2.5-MDa human proteasome in its ground state, as well as subnanometer-resolution structures of the holoenzyme in three alternative conformational states. The substrate-unfolding AAA-ATPase channel is narrowed by 10 inward-facing pore loops arranged into two helices that run in parallel with each other, one hydrophobic in character and the other highly charged. The gate of the core particle was unexpectedly found closed in the ground state and open in only one of the alternative states. Coordinated, stepwise conformational changes of the regulatory particle couple ATP hydrolysis to substrate translocation and regulate gating of the core particle, leading to processive degradation.
Indexed by:Journal paper
Discipline:Natural Science
Document Type:J
Volume:113
Issue:46
Page Number:12991-12996
Translation or Not:no
Date of Publication:2016-11-15
Included Journals:SCI
First Author:Shuobing Chen,Jiayi Wu,Ying Lu
Correspondence Author:Marc W Kirschner,Youdong Mao
All the Authors:Yong-Bei Ma,Byung-Hoon Lee,Zhou Yu,Qi Ouyang,Daniel J Finley
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